Apparatus for rapid immunoassays

Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition

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422 58, 436177, 436809, 435287, B01L 1100

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active

052195287

ABSTRACT:
An apparatus for use in a ligand-receptor assay procedure for the quantitative determination of the concentration of a target ligand in a liquid sample. The apparatus contains top and middle plates having holes therethrough. The holes in the two plates are in axial alignment and the holes in the middle plate have sidewalls that extend below the bottom surface of the middle plate. The apparatus contains a bottom chamber having an opening on the upper side thereof facing the bottom surface of said middle plate. The chamber contains at least one port extending through a surface thereof to permit a vacuum to be created within said chamber and means for accepting a microtiter plate containing a plurality of wells. When the microtiter plate is positioned within the chamber, the ends of the holes extending beneath the bottom surface of the middle plate are located within the wells. The three members of the apparatus can be secured together in vacuum tight relationship such that when a liquid permeable membrane is placed between the top and middle plates, liquid is placed in the holes in the top plate and vacuum is created in the chamber, the liquid is drawn at a controlled rate directly through the membrane without lateral dispersion, through the holes in the middle plate and, in turn, into the chamber.

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Iisselmuiden et al., European Journal of Clinical Microbiology, vol. 6, No. 3 (1987) pp. 281-285.

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