Chemistry: physical processes – Physical processes – Crystallization
Patent
1975-04-07
1977-02-22
Wolk, Morris O.
Chemistry: physical processes
Physical processes
Crystallization
G01N 3106, G01N 3316
Patent
active
040090051
ABSTRACT:
Apparatus for radioimmunoassay provides a continuously reusable short cycle analytical system of particular use in biochemical analysis such as the determination of steroids, hormones, polypeptides, viruses, vitamins, drugs, and the like. The apparatus disclosed samples a mixture of an unlabelled antigen sample and a known concentration of labelled antigen, bringing the mixture into contact with a mass of appropriate antibody that has been immobilized thereby to bind at least part of the mixed antigen to the antibody. The amount of bound and/or unbound labelled antigen is detected and counted and, by use of previously prepared standards, the unknown concentration of unlabelled antigen is found. The still immobilized antibody is regenerated for immediate reuse by rinsing it with a selected eluting solution that is capable of breaking the antigen-antibody. Alternate ways of immobilizing the antibody are described. Several suitable eluting solutions are disclosed. Also disclosed is a novel apparatus by which immunoassay may be automatically carried out and the antibody reused many times. By proper substitution, the antibody and antigen may be reversed.
REFERENCES:
patent: 3519390 (1970-07-01), Dickey et al.
patent: 3607073 (1971-09-01), Stamm
patent: 3788812 (1974-01-01), Dupre
patent: 3793445 (1974-02-01), Updike et al.
patent: 3806321 (1974-04-01), Durrum et al.
patent: 3814581 (1974-06-01), Reunanen
Chem. Abstr., vol. 74:123000K (1971).
Chem. Abstr., vol. 75:33018p (1971).
Updike et al., Clin. Chem., vol. 19, pp. 1339-1343 (1973).
Akanuma et al., Biochem. Biophys. Res. Comm., vol. 38, pp. 947-953 (1970).
Becton Dickinson and Company
Hagan Timothy W.
Martella Mario A.
Wolk Morris O.
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