Radiant energy – Luminophor irradiation
Patent
1994-01-31
1995-05-23
Dzierzynski, Paul M.
Radiant energy
Luminophor irradiation
2504591, G01N 2164
Patent
active
054183715
ABSTRACT:
A quantitative fluorometer for multiple fluorophores having dual time-modulated beams of excitation light. Each beam is synchronized with a separate detector and lock-in amplifier. The fluorophores are simultaneously excited and the combined fluorescent emission is resolved into components corresponding to each fluorophore. Confocal scanning means are used to excite and detect fluorescent emission from locations throughout a volume. The location specific output of each amplifier is stored in a computer which resolves the emission into the components corresponding to each fluorophore. The location specific data may be further processed or visually displayed. Multiple amplifiers for each detector channel allow phase discrimination in each channel so that prompt and delayed fluorescence may be measured, allowing use of multiple fluorophores in each detector channel.
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Aslund Nils R. D.
Carlsson Kjell S.
Dzierzynski Paul M.
Henig Richard
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