Apparatus for purifying islets of Langerhans

Details Apparatus for purifying islets of Langerhans Apparatus for purifying islets of Langerhans

1992-03-18

1993-12-28

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435311, 210800, 210513, C12M 100

Patent

active

052739040

ABSTRACT:
A method and apparatus to concentrate and purify islets of Langerhans from a tissue suspension containing islets and tissue fragments. The tissue suspension is flowed through an inclined channel such that laminar flow is established in the channel. The islets settle toward the channel bottom and are drawn out of the channel through an outlet in the channel bottom, while the remaining tissue suspension flows out a second outlet positioned higher than the islet outlet. Additional concentration or purification may be accomplished by passing the islets through the channel additional times and by centrifuging or filtering operations.

REFERENCES:
patent: 2009559 (1935-07-01), Mieder
patent: 2142888 (1939-01-01), Donnallan
patent: 4424132 (1984-01-01), Iriguchi
patent: 4765899 (1988-08-01), Wells et al.
patent: 4868121 (1989-09-01), Scharp et al.
patent: 5079160 (1992-01-01), Lacy et al.

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