Apparatus for pretreating cells for flow cytometry

Details Apparatus for pretreating cells for flow cytometry Apparatus for pretreating cells for flow cytometry

1990-09-11

1992-12-01

Housel, James C.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Sample mechanical transport means in or for automated...

422 64, 422 72, 422 81, 422100, 436 45, 436177, G01N 930, G01N 2100

Patent

active

051679264

ABSTRACT:
A plurality of open-top sample tubes, each containing a simple liquid containing cells to be treated, are removably mounted on a disklike sample carrier which is rotatable about a vertical axis for centrifuging the samples. Disposed above the sample carrier are a set of fixed reagent supply nozzles for dropping a desired reagent or reagents into the sample tubes and at least one supply-discharge nozzle movable into and out of the successive sample tubes. A hydropneumatic circuit is coupled to the supply-discharge nozzle for intimately intermingling the samples and the reagents and for rinsing the supply-discharge nozzle and other required parts. In an alternate embodiment a single supply-discharge nozzle is employed for reagent supply into the sample tubes, for intermingling the samples and the reagents, and for rinsing.

REFERENCES:
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patent: 4236666 (1980-12-01), Aeschlimann et al.
patent: 4323537 (1982-04-01), Mody
patent: 4332768 (1982-06-01), Berglund
patent: 4708940 (1987-11-01), Yoshida et al.
patent: 4837160 (1989-06-01), Meserol et al.
patent: 4845025 (1989-07-01), Lary et al.
patent: 4980130 (1990-12-01), Metzger et al.
patent: 4983359 (1991-01-01), Tomioka et al.

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