Apparatus for preparing single cell suspension

Details Apparatus for preparing single cell suspension Apparatus for preparing single cell suspension

1982-02-01

1983-11-01

Jones, Raymond N.

Chemistry: molecular biology and microbiology

Apparatus

Including condition or time responsive control means

435299, 435311, 2104161, 241283, C12M 302, C12M 116, B02C 100, B02D 3300

Patent

active

044130597

ABSTRACT:
A method and apparatus for preparing a liquid suspension of single, independent tissue cells for, e.g., subsequent cell proliferation as by in vitro culturing, is disclosed. The method involves introducing the combination of subdivided tissue and liquid medium into a chamber defined in part by a foraminous wall portion, and contracting the chamber to force the tissue through the foraminous wall, thereby further subdividing the tissue. An apparatus embodiment includes the combination of confining means for confining a liquid suspension of tissue; a screen; means supporting the screen; and pump means for passing the liquid suspension of tissue from the confining means back and forth through the screen.

REFERENCES:
patent: 2437818 (1948-03-01), Hetzler
patent: 3493503 (1970-02-01), Mass

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