Apparatus for multi-zone polymerase chain reaction

Details Apparatus for multi-zone polymerase chain reaction Apparatus for multi-zone polymerase chain reaction

1998-12-23

2000-10-31

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Apparatus

Mutation or genetic engineering apparatus

435 6, 435 911, 435 912, 536 231, 536 243, 536 2433, C12M 100, C12Q 168, G01N 3353, C12P 1934, C07H 2104

Patent

active

061401102

ABSTRACT:
A process of amplifying a nucleic acid sequence by a procedure involving a polymerase chain reaction or a ligase chain reaction. The process involves repeated cycles of steps including a nucleic acid denaturing step and a nucleic acid synthesis step, the synthesis step being carried out under the action of an enzyme (a nucleic acid polymerase or ligase). The denaturing step and the synthesis step are carried out in different denaturing and synthesis reaction zones, respectively, and, during the repeated cycles, the enzyme is maintained in isolation from the denaturing reaction zone, and conditions or reagents required for the denaturing step are maintained in isolation from the synthesis reaction zone to the extent that the reagents and conditions required for denaturing do not impede the synthesis reaction to a substantial extent. The use of separate zones for the steps of the reactions means that an enzyme that is destroyed or degraded by the reagents and conditions required for denaturing (e.g. a thermolabile or alkalolabile polymerase or ligase) may be used in the reaction. Moreover, the use of multiple zones means that inexpensive equipment may be used for the process.

REFERENCES:
patent: 5333675 (1994-08-01), Mullis et al.
patent: 5364591 (1994-11-01), Green et al.
patent: 5863502 (1999-01-01), Southgate
patent: 5912129 (1999-06-01), Vinayagamoorthy et al.
patent: 5985651 (1999-11-01), Hunicke-Smith

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