Apparatus for microbiological analysis of biological samples in

Details Apparatus for microbiological analysis of biological samples in Apparatus for microbiological analysis of biological samples in

1993-04-07

1994-10-18

Czaja, Donald E.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435284, 435296, 435300, 435808, 436164, 436165, C12M 134

Patent

active

053568150

ABSTRACT:
An apparatus for microbiological analysis of biological samples in liquid suspension by light-scattering technique, which employs test-tube containers (11) made of a material transparent to electromagnetic radiations of a determined wave length. Each container (11) cooperating with a thermostat system (17) and at least momentarily with a stirring assembly (31) and relative keeper stirring means (18). The container (11) cooperating with a focussing and collimation system (22) and with a monitoring device (24), is illuminated by electromagnetic radiation of a determined wave length (74) emitted with its own axis (29) of emission and transmitted through the container (11) by the focussing and collimation system (22). The radiation is scattered by any microorganisms present in the sample with an intensity related to their number and morphology. This scattered radiation is picked-up in succession with desired timing by the monitoring device (24) according to determined angles, from 0.degree. to 180.degree., in relation to the emission axis (29). A computer collects the resulting data and constructs a curve representing intensity of scattered radiation versus time. The curve parameters are compared with reference values contained in a data bank of the computer (13) for determination of the typical parameters of the analysis i.e., the counting, the replication rate and the morphology of microorganisms present in the sample.

REFERENCES:
patent: 5084629 (1992-01-01), Petralli
patent: 5104221 (1992-04-01), Bott et al.
patent: 5155543 (1992-10-01), Hirako

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