Apparatus for methods for preserving, transporting storing, re-h

Details Apparatus for methods for preserving, transporting storing, re-h Apparatus for methods for preserving, transporting storing, re-h

1991-07-22

1992-10-13

Warden, Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

435260, 435296, 435297, 435298, 435810, C12N 100

Patent

active

051550393

ABSTRACT:
The present invention is directed to systems and methods for preserving, transporting, storing, re-hydrating and delivering viable micro-organisms. More particularly, the present invention is directed to a kit and method for preserving and storing dried, microbiological organisms and for re-hydrating and delivering specific and reproducible numbers of viable organisms therefrom. The kit and method of the present invention provides the capability to deliver a known quantity of rehydrated micro-organisms as a single, total dose of cells without the inaccuracies and safety hazards associated with standard needle, syringe or pipette transfer systems. The kit includes first and second vial and cap combinations, the first carrying the dried organisms in a dry, biologically inert atmosphere, the second carrying a pre-measured quantity of re-hydrating fluid. A quantitative number of selected microbiological organisms are dried under mild conditions, i.e., ambient temperature and pressure using forced air flow, to fixative sites on the underside of the first cap engaged in sealing relation with the first vial. Transfer of that cap to the second vial containing the re-hydrating fluid provides easy re-hydration and the ability to deliver specific and reproducible numbers of viable organisms after extended storage periods at normal refrigeration temperatures.

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