Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus
Patent
1987-12-07
1989-07-25
Makay, Albert J.
Chemistry: molecular biology and microbiology
Apparatus
Differentiated tissue perfusion or preservation apparatus
435285, 435296, C12M 300
Patent
active
048513547
ABSTRACT:
An apparatus useful for studying cells in culture under conditions which reproduce their natural, in vivo mechanical environment is disclosed. The device comprises an airtight well having an optically transparent compliant base of a biologically compatible material on which said cells may be grown and an optically transparent, removable cap, coupled with a ported, airtight reservoir which reservoir has an optically transparent base and which reservoir can be filled with pressurizing media to create cyclic variations in hydrostatic pressure beneath said compliant base, causing said compliant base to deform and thereby exert a substantially uniform biaxial strain on the cells attached thereto.
REFERENCES:
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Winston, F. K., et al., Abstract of a paper entitled "The In Vitro Response of Endothelium to Mechanical Loading," 38th ACEMB in Chicago, Ill. from 9/30-10/2/85.
Thibault, L. E. and Fry, L., "Mechanical Characterization of Membranelike Biological Tissue," J. Biomech. Eng. 105:31-38 (1983).
Davies, Peter F., et al., "Influence of Hemodynamic Forces on Vascular Endothelial Function," J. Clin. Invest., 73:1121-1129 (4/1984).
Leung, Donald Y. M., et al., "Cyclic Stretching Stimulates Synthesis of Matrix Components by Arterial Smooth Muscle Cells In Vitro," Science, 191:475-577 (1976).
Gorfien, S. F., et al., Abstract of a paper entitled "Fibronectin and Type III Collagen Production by Bovine Pulmonary Artery Endothelium Subjected to Cyclic Biaxial Strain," Cell Bio Meeting in Washington, D.C. on 12/7/86.
Winston, F. K., et al., Paper entitled "Response of Endothelial Cells in Culture to Biaxial Deformation," Northeast Bioengineering Conference in Phila. on 3/87.
B. E. Sumpio, et al., "Mechanical Stress Stimulates Aortic Endothelial Cells to Proliferate," Journal of Vascular Surgery, 1987; 6:252-6.
B. E. Sumpio, et al., "Alterations in Aortic Endothelial Cell Morphology and Cytoskeletal Protein Synthesis During Cyclic Tensional Deformation," Journal of Vascular Surgery, 1988; 7:130-8.
A. J. Banes, et al., "A New Vacuum-Operated Stress-Providing Instrument that Applies Static or Variable Duration Cyclic Tension or Compression to Cells in Vitro," J. Cell Sci. 75, 35-42 (1985).
Sales Literature of Flexcell Corp., Apr. 1988.
Macarak Edward J.
Thibault Lawrence E.
Winston Flaura K.
Kamen Noah
Makay Albert J.
Trustees of the University of Pennsylvania
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