Apparatus for measuring velocity of enzyme reaction

Details Apparatus for measuring velocity of enzyme reaction Apparatus for measuring velocity of enzyme reaction

1985-03-05

1986-11-04

Cohan, Alan

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

356417, 422 52, C12M 134

Patent

active

046210598

ABSTRACT:
An enzyme is immobilized to a capillary column, and a solution which contains a chemical substance and a luminescent substance flows through the column. A plurality of optical fibers are arranged along the longitudinal direction of the column, and the luminescence in the column is introduced to a photodiode array through the fibers. The distribution of the luminescent intensity along the longitudinal direction of the column is detected by the photodiode array. Thus, the rate of quantity increase of the product produced by the enzyme reaction can be measured while the solution is flowing through the column, thereby producing the enzyme activity by the end assay and the rate assay.

REFERENCES:
patent: 3413464 (1968-11-01), Kamentsky
patent: 4021307 (1977-05-01), Mosbach
patent: 4537861 (1985-08-01), Elings
patent: 4563331 (1986-01-01), Losee

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