Apparatus for measuring two different fluorescences of a sample

Details Apparatus for measuring two different fluorescences of a sample Apparatus for measuring two different fluorescences of a sample

1974-10-10

1976-07-27

Borchelt, Archie R.

Radiant energy

Luminophor irradiation

250461B, G01J 158

Patent

active

039719510

ABSTRACT:
In apparatus, such as a fluorometric microscope, for measuring two different fluorescences in a sample, the sample is irradiated by light modulated at a first frequency and having a first wavelength for causing a first fluorescence and also is irradiated by light modulated at a second frequency different from the first frequency and having a second wavelength for causing a second fluorescence. A photoelectric element is responsive to the light emitted by the sample during both fluorescences for generating an electrical signal representative of the combined intensities of the two fluorescences. From this electrical signal, a demodulator derives first and second signal components having the first and second frequencies, respectively, and having amplitudes representative of the individual intensities of the first and second fluorescences, respectively.

REFERENCES:
patent: 3825762 (1974-07-01), White
patent: 3832555 (1974-08-01), Ohnishi

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