Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Patent
1991-12-16
1993-12-14
Warden, Robert J.
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
422 8207, 422 8208, 436172, 2504581, 356307, G01J 330
Patent
active
052707881
ABSTRACT:
An improved method and apparatus for measuring polarized fluorescence emissions compensates for background emissions without separating a fluorescing material from background material. The method involves measuring the horizontally and vertically polarized components of the fluorescence emission at a primary wavelength and at least one secondary wavelength selected on the basis of the bathochromic shift of the spectrum of the fluorescence emissions from the fluorescing material as compared to the fluorescence emissions from the background. From these measurements, a factor representing the fraction of the total intensity of fluorescence emissions due to background fluorescence is determined. From this factor, the intensities of the vertically and horizontally polarized fluorescence emissions due to background fluorescence are derived and subtracted from the measurements at the primary wavelength to obtain intensities due solely to the material being analyzed.
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Cercek Boris
Cercek Lea
Thornton Krisanne M.
Warden Robert J.
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