Apparatus for measuring lymphocyte subclasses

Details Apparatus for measuring lymphocyte subclasses Apparatus for measuring lymphocyte subclasses

1987-09-14

1991-01-08

Warden, Robert J.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing liquid or solid sample

356 39, 356 73, 435808, 436 56, 436546, 436805, 436808, G01N 2100, G01N 3300, G01N 33533

Patent

active

049833596

ABSTRACT:
A blood sample is prepared by combining a fluorescently tagged monoclonal antibody with blood cells. The sample is then passed through a flow cytometer and light scattered by each blood cell in two directions is sensed simultaneously by a single sensor. Lymphocytes are discriminated from other cells by using an intensity distribution of the total scattered light. Then, by using a distribution of fluorescent light intensity of each cell thus discriminated, the cells in a lymphocyte subclass of interest are enumerated with a high accuracy. The data acquired are utilized in diagnosing immunological incompetence or the like.

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