Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lipoproteins – e.g. – egg yolk proteins – cylomicrons – etc.
Reexamination Certificate
2008-06-02
2010-11-23
Kam, Chih-Min (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lipoproteins, e.g., egg yolk proteins, cylomicrons, etc.
C530S412000, C530S419000, C422S082010
Reexamination Certificate
active
07838631
ABSTRACT:
The present invention provides a method of separating lipoproteins other than high density lipoproteins from a biological fluid. The method can quickly measure HDL cholesterol with a simple configuration and without the need of providing additional complicated devices. In this method, high density lipoproteins not generating any precipitate are fractionated from low density lipoproteins, very-low density lipoproteins, and chylomicrons generating precipitates. Then the precipitates are removed not by centrifugal separation based on the conventional technology, but by filtration using a filter to separate high density lipoproteins in blood serum. A hydrophilic cellulose-mixed ester is preferable as a material for the filter, and the pore diameter is 0.8 μm or below. When the filtering method is employed, it is possible to eliminate the complicated operations required in the conventional centrifugal separation, and to shorten the time it takes for separation of the high density lipoproteins.
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European Patent Office, “European Search Report” for European Application No. 08 01 0052, Mar. 10, 2009, 9 pages.
Miyake Masafumi
Yamashita Kotaro
Hitachi High-Technologies Corporation
Kam Chih-Min
Mattingly & Malur, P.C.
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