Apparatus for isolation of synthetic peptide without mechanical

Details Apparatus for isolation of synthetic peptide without mechanical Apparatus for isolation of synthetic peptide without mechanical

1993-02-24

1994-10-18

Warden, Robert J.

Chemical apparatus and process disinfecting, deodorizing, preser

Chemical reactor

Organic polymerization

422 99, 422101, 935 87, 935 88, 435287, C08F 200, B01L 300, C12M 100

Patent

active

053565967

ABSTRACT:
In connection with an automated peptide synthesizer, an apparatus and associated method through which peptides produced in simultaneous multiple synthesis are together isolable. Cleavage to liberate the peptides from linkage to the support matrix of the solid phase is carried out in the same vessels in which the synthesizing reactions took place. Prior to cleavage, a drainage port of each reaction vessel is closed off by a cap. The reaction vessel is inserted into a centrifuge tube, which in turn is put into a rack supporting a number of tubes equal to the number of channels of the peptide synthesizing apparatus. After the peptides are cleaved and dissolved into cleaving solution added into each of the reaction vessels, the caps are removed from the drainage ports of each reaction vessel, and the vessel is returned into the centrifuge tube. A plastic jet-fitting attached to the nozzle tip of a blow unit pressure gun is inserted into and pressed into contact against the supply opening of the reaction vessel. Operating a trigger of the pressure gun releases pressurized inert gas into the reaction chamber. The peptide-dissolved cleaving solution is thereby passed through the drainage port, and is thus transferred in liquid phase into the centrifuge tube as filtrate. Associated with the blow unit pressure gun, a needle tube fitting is provided for localized desiccation of wetted peptide following precipitation and centrifuging.

REFERENCES:
patent: 3557077 (1971-01-01), Brunfeldt et al.
patent: 3700174 (1972-10-01), Beck
patent: 3777981 (1973-12-01), Probst et al.
patent: 4362699 (1982-12-01), Verlander et al.
patent: 4483964 (1984-11-01), Urdea et al.
patent: 4598871 (1986-07-01), Hartle
patent: 4746490 (1988-05-01), Saneii
patent: 4766082 (1988-08-01), Marteau D'Autry
patent: 4775629 (1988-10-01), Kuhl et al.
patent: 4850536 (1989-07-01), Teranishi et al.
patent: 5019348 (1991-05-01), Ohms et al.
patent: 5055272 (1991-10-01), Wheeler et al.
patent: 5170940 (1992-12-01), Salber et al.
patent: 5288464 (1994-02-01), Nokihara
Cole-Palmer Products Catalog, 1993-1994, p. 829, 1992.

Affiliated with

Also associated with

Rating

Apparatus for isolation of synthetic peptide without mechanical does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Apparatus for isolation of synthetic peptide without mechanical , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus for isolation of synthetic peptide without mechanical will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2370406