Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is particulate and the particles are of...
Patent
1992-10-30
1994-08-30
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is particulate and the particles are of...
422 58, 422 72, 422101, 435296, 436164, 436165, 436514, 436518, 436528, 436531, 436534, 436536, 436538, 436541, 436805, 436810, 436824, 436829, G01N 33536
Patent
active
053427904
ABSTRACT:
A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labeled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labeled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labeled antibodies will thus not interfere with the analysis.
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Pharmacia LKB Biotechnology Products, 1988.
Levine Robert A.
Mercolino Thomas J.
Recktenwald Diether J.
Terstappen Leon W. M. M.
Wardlaw Stephen C.
Becton Dickinson and Company
Chin Christopher L.
Jones William W.
Scheiner Toni R.
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