Apparatus for enzyme detection

Details Apparatus for enzyme detection Apparatus for enzyme detection

1978-02-27

1981-01-06

Warden, Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435 4, 435 17, 435 21, 435 26, 435291, 435803, 435808, 435815, 2101982, 210656, 422 59, C12M 140, C12M 134

Patent

active

042437539

ABSTRACT:
An apparatus is disclosed for enzyme detection that is particularly well suited for high performance liquid chromatography. The apparatus is basically a flow-through enzyme detector that is capable of maintaining zero-order reaction kinetics with respect to substrate during enzyme detection and this results in a linear accumulation of product that can be readily measured to provide a dependable readout of detected enzymes. A sample in liquid form that may include one or more enzymes is coupled through a separation column to separate the enzymes, after which a reaction causing substrate is added and the resulting mixture is caused to flow through a reaction chamber, reaction products then being detected with the readout therefrom indicating the detected enzymes. The dimensions of the reaction chamber are dependent upon flow rates and must be selected to allow the mixture to remain in the chamber for a preselected reaction time. In addition, the chamber and packing material therein are selected to assure minimization of chromatographic profile distortion by band spreading as well as preventing separation of the components of the mixture passing therethrough. To this end, the chamber and packing material within the chamber must be of non-porous material, inert to proteins to be detected, and must assure even flow conditions.

REFERENCES:
patent: 3170969 (1965-02-01), Lerner et al.
patent: 3468637 (1969-09-01), Hammond
patent: 3493218 (1970-02-01), Castellucci
patent: 3722181 (1973-03-01), Kirkland et al.
patent: 3796657 (1974-03-01), Pretorius et al.
patent: 3808125 (1974-04-01), Good
patent: 3838011 (1974-09-01), Hagen et al.
patent: 3956065 (1976-05-01), Idaszak et al.
patent: 3983299 (1976-09-01), Regnier

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