Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1995-06-06
2000-08-15
Wilson, James O.
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204456, 204607, 204608, 204609, 204612, 435291, 435173, 435287, 435289, 935 52, 935 89, 935 93, B01D 6142, B01D 5702
Patent
active
061030848
ABSTRACT:
An apparatus for electroporation stores a level of charge, specified by a user, on a capacitor, which is delivered to a cuvette through an optically isolated high voltage switch. The capacitor is charged through a charging system, including a current mode pulse width modulation control circuit, which monitors the current in the primary winding of a transformer and supplies a pulse width modulated signal, limiting the current on every pulse to a level set by the microcontroller, to the controlling transistor in order to generate the drive to the primary winding of the transformer. A controlled amount of energy is transferred through each pulse to the capacitor. The microcontroller monitors the voltage on the capacitor up to a threshold level to predict the number of pulses necessary to store the requested amount of charge on the capacitor. The microcontroller will then count the number of pulses until the number of pulses necessary to store the requested amount of charge on the capacitor has been reached. At this point the requested voltage is delivered to the cuvette through the optically isolated high voltage switch comprised of sensitive gate SCRs coupled together in series. The voltage and current at the cuvette are monitored and input to the microcontroller so that the impedance at the cuvette is calculated by the microcontroller and errors in the composition of the solution within the cuvette are detected.
REFERENCES:
patent: 3898410 (1975-08-01), Peters, Jr.
patent: 4081340 (1978-03-01), Zimmermann et al.
patent: 4571535 (1986-02-01), Gyugyi
patent: 4750100 (1988-06-01), Ragsdale
patent: 4848355 (1989-07-01), Nakamura et al.
patent: 4910140 (1990-03-01), Dower
patent: 4946793 (1990-08-01), Marshall, III
patent: 5186800 (1993-02-01), Dower
patent: 5676646 (1997-10-01), Hofmann et al.
Machl, T., and Robinson, J.K., "Comparison of Electrical Versus Chemical Transformation Techniques," Biomedical Products, (May 1995).
"SCR Manual," General Electric, Fifth Edition, pp. 149-153, 158-159, 162-165, and 444-447;.
"Electrostatic Discharge Simulator Model ESS-630A," Noise Laboratory Ltd.;.
Alberts, Bruce, et al., "Molecular Biology of the Cell," Garland Publishing Inc., New York, p. 923 (1983);.
Davis, Bernard D. et al, "Microbiology," Harper & Row, pp. 34 and 878 (1968);.
MacNeil, Douglas J., "Introduction of Plasmid DNA into Streptomyces lividans by Electroporation," FEMS Microbiol. Lett. 42:239-244 (1987);.
Miller, Jeff F. et al., "High-Voltage Electroporation of Bacteria: Genetic Transformation of Campylobacter jejuni with Plasmid DNA," Proc. Natl. Acad. Sci. USA 85:856-860 (1988);.
Shivarova, N., et al., "Microbiological Implications of Electric Field Effects," Zeitschrift Allge. Mikro. 23(9):595-599 (1983);.
Potter, Huntington, et al., "Enhancer-Dependent Expression of Human .kappa. Immunoglobulin Genes Introduced into Mouse Pre-B Lymphocytes by Electroporation," Proc. Natl. Acad. Sci. USA 81:7161-7165 (1984);.
Dower, Bill, "Electro-Transformation of Intact Bacterial Cells," Molecular Biology Reports 1(1): p5 (1987);.
Harlander, Susan K., "Transformation of Streptococcus lactis by Electroporation," Streptococal Genetics, pp. 229-233 (1987);.
Chassy, Bruce M., et al., "Transformation of Lactobacillus casei by Electroporation," FEMS Microbiol. Lett. 44: 173-177 (1987);.
Powell, Ian B., et al., "A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation," Appl. Environ. Microbiol. 54(3):655-660 (1988);.
BioRad, "Gene Pulser.TM. Transfection Apparatus Operating Instructions and Applications Guide" pp. 1-28 (Jan. 1987).
Eppendorf Netheler-Hinz GmbH
Wilson James O.
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