Apparatus for electrophoretic recovery of nucleic acids and othe

Details Apparatus for electrophoretic recovery of nucleic acids and othe Apparatus for electrophoretic recovery of nucleic acids and othe

1984-04-06

1985-10-08

Metz, Andrew H.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204299R, 2041828, G01N 2728

Patent

active

045458880

ABSTRACT:
The invention is an improved electrophoresis device for the recovery of nucleic acids and other substances. The apparatus and method of this invention is for the purpose of recovering large charged molecules in a pure state after they have been separated from a mixture by gel electrophoresis; the charged molecules from gels which has high quantitative recovery without contamination by an apparatus and method which is rapid and convenient to use. The apparatus consists of a plurality of transfer chambers suitably supported in a vessel for containing an aqueous buffer solution, a plurality of filter discs for support of a layer of DEAE cellulose resin in the bottom of the transfer chambers, a plurality of negative electrodes (one in each of the upper portion of each of the transfer chambers), a positive electrode for placement in the buffer which will surround the plurality of transfer chambers, and a power supply.

REFERENCES:
patent: 3384564 (1968-05-01), Ornstein et al.
patent: 3791950 (1974-02-01), Allington
patent: 3795600 (1974-03-01), Allington
patent: 3847785 (1974-11-01), Allington
patent: 3867271 (1975-02-01), Hoefer
patent: 4048049 (1977-09-01), Hoefer
patent: 4284491 (1981-08-01), Vesterberg

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