Apparatus for electrophoresis separation

Details Apparatus for electrophoresis separation Apparatus for electrophoresis separation

1977-06-27

1978-05-09

Prescott, Arthur C.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204180G, G01N 2726, G01N 2728

Patent

active

040885615

ABSTRACT:
An apparatus is disclosed for simultaneously performing electrophoresis separations on a plurality of slab gels containing samples of protein, protein subunits or nucleic acids. A reservoir of buffer solution is divided into three compartments by two parallel partitions having vertical slots spaced along their length. A sheet of flexible, electrically insulative material is attached to each partition and is provided with vertical slits aligned with the slots. Slab-gel holders are received within the slots with the flexible material folded outwardly as flaps from the slits to overlay portions of the holder surfaces and thereby act as electrical and liquid seals. An elongated, spaghetti-like gel containing a sample of specimen that was previously separated by isoelectric focusing techniques is vertically positioned along a marginal edge portion of the slab gel. On application of an electrical potential between the two outer chambers of buffer solution, a second dimensional electrophoresis separation in accordance with molecular weight occurs as the specimen molecules migrate across the slab gel.

REFERENCES:
patent: 3255100 (1966-06-01), Raymond
patent: 3719580 (1973-03-01), Roberts et al.
patent: 3751357 (1973-08-01), Rains
patent: 3847788 (1974-11-01), Wallace
patent: 3888758 (1975-06-01), Saeed

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