Apparatus for electrofusion of biological particles

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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204302, B01D 5702

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active

044760043

ABSTRACT:
Apparatus and methods of use thereof are provided for fusing neutral polarizable biological bodies and sorting neutral polarizable biological bodies by imposing them to a non-uniform electric field. The dielectrophoretic effect of the non-uniform electric field will either impose variable displacements of the types of cells to facilitate sorting, or will align cells contiguously between a pair of electrodes in preparation for fusion. To fuse the cells the aligned cells are exposed to a direct current impulse.

REFERENCES:
patent: 3162592 (1964-12-01), Pohl
patent: 4326934 (1982-04-01), Pohl
Zimmermann, U., et al, "Fusion of Avena Sativa Mesophyll Gell Protoplasts by Electrical Breakdown", Biochimica et Biophysica Acta, 641, (1981), 160-165.
Pohl, H. A., et al, "The Continuous Positive and Negative Dielectrophoresis of Microorganisms", J. Bio. Phys., 9, 67-86, (1981).
Zimmermann, U., et al, "Electric Field-Induced Cell-To-Cell Fusion", J. Membrane Biol., 67, 165-182, (1982).
Vienken, J., et al, "Electric Field-Induced Fusion: Electro-Hydraulic Procedure for Production of Heterokaryon Cells in High Yield", Fed. Eur. Biomed. Soc. Lett., 137, 11-13, (1982).
Crane, J. S., et al, "Use of the Balanced-Cell Technique to Determine Properties of Single Yeast Cells", J. Biol. Phys., 5, 49-73, (1978).
Bischoff, et al, "Human Hybridoma Cells Produced by Electro-Fusion", Fed. Eur. Biochem. Soc. Lett., 147, 64-68, (1982).
Halfmann, H. H., et al, "Transfer of Mitochondrial Function into a Cytoplasmic Respiratory-Deficient Mutant of Saccharomyces Yeast by Electro-Fusion", Current Genetics, 6, 25-28, (1982).
Hub, H. H., et al, "Preparation of Large Unilamellar Vesicles", Fed. Eur. Biochem. Soc. Lett., 140, 254-256, (1982).
Zimmermann, U., et al, "Electric Field-Induced Release of Chloroplasts from Plant Protoplasts", Naturwissen, 69, 451, (1982).
Zimmermann, U., et al, "Electric Field-Mediated Cell Fusion", J. Biol. Phys, 10, 43-50, (1982).
Changben, L., et al, "Use of Human Erythrocyte Ghosts for Transfer of .sup.125 I-BSA and .sup.125 I-DNA into Animal Cells from Cell Fusion", Scientia Sinica (Series B), 25, 680-865, (1982).
Neumann, B., et al, "Cell Fusion Induced by High Electrical Impulses Applied to Dictyostelium", Naturissen, 67, 414, (1980).
Murch, A. R., et al, "Direct Evidence That Inflammatory Multinucleate Giant Cells Form by Fusion", Pathol. Soc. Gr. Brit. Ire., 137, 177-180, (1982).
Zimmermann, U., "Cells with Manipulated Functions: New Perspectives for Cell Biology, Medicine, and Technology", Angew, Chem. Int. Ed. Engl., 20, 325-344, (1981).
Chen, C. S., et al, "Biological Dielectrophoresis: The Behavior of Lone Cells in a Nonuniform Electric Field", Ann. N.Y. Acad. Sci., 238, 176-185, (1974).
Kaler, K., et al, "Dynamic Dielectrophoretic Levitation of Living Individual Cells", J. Biol. Phys., 8, 18-31, (1980).
Weber, H., et al, "Enhancement of Yeast Protoplast Fusion by Electric Field Effects", a preprint for Proceedings of the Fifth International Symposium on Yeasts, London, Ontario, Canada, (Jul. 1980).
Pohl, H. A., et al, "Continuous Dielectrophoretic Separation of Cell Mixtures", Cell Biophys., 1, 15-28, (1979).
Pohl, H. A., et al, "Dielectrophoretic Force", J. Biol. Phys., 6, 133, (1978).
Pohl, H. A., "Biophysical Aspects of Dielectrophoresis", J. Biol. Phys., 1, 1-16, (1973).

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