Apparatus for distinguishing multiple subpopulations of cells

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Chemiluminescent

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2504612, 422 68, 436 63, 436172, G01N 2164, G01N 3350

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active

044990529

ABSTRACT:
A method of distinguishing multiple subpopulations of cells from a single sample of cells of a variety of types comprises labeling particles with two or more marking agents. These particles are marked in a plurality of different pre-selected ratios of the agents ranging between zero percent and one hundred percent of each agent. Each such agent has distinguishing, quantifiable marking characteristics. The differently labeled particles are mixed with cells suspected of having specific receptors for the differently labeled particles. Each cell is analyzed to determine the ratio of any two identifiable marking characteristics associated with each cell so that it can be classified in a subpopulation category if its ratio of marking characteristics is related to one of the pre-selected ratios of marking agents.
An apparatus for carrying out the above-described method is also within the purview of the present invention.

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Latt et al., Journal of Histochemistry and Cytochemistry, vol. 27, No. 1 pp. 65-71, 1979.
Clausen et al., International J. Andrology Suppl. 2 (2) 1978, pp. 513-522.

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