Apparatus for direct blotting and automated electrophoresis, tra

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, G01N 2726, G01N 27447

Patent

active

052345599

ABSTRACT:
A separation and direct blot transfer module useful in gel electrophoresis is disclosed in which a transfer membrane stabilized by a frame is moved substantially orthogonally relative to the plane of the separation gel while in minimal contact with the end of the gel toward which molecular fragments are driven electrophoretically. The frame moves at a controlled velocity in a selected direction away from a line of contact that generates a controlled tension in the membrane which is a function of the loading on the line of contact. The module is particularly amenable to automated processing. Apparatus for automated separation, transfer, membrane processing, detection and identification is disclosed in which the module of the invention is employed.

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