Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Reexamination Certificate
2005-03-22
2005-03-22
Wallenhorst, Maureen M. (Department: 1743)
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
C422S082010, C422S082020, C422S082050, C422S082090, C436S063000, C436S008000, C436S010000, C436S164000, C436S149000, C436S150000, C435S002000
Reexamination Certificate
active
06869569
ABSTRACT:
Blood cells of interest are readily distinguishable from other blood cells and look-a-like particles found in a blood sample by their back-scatter signature. A preferred method for differentiating platelets in a blood sample is to irradiate the cells and particles, one at a time, with a beam of radiation, and to detect back-scattered (reflected) radiation using a plurality of optical fibers to transmit the back-scattered radiation to a high-gain photodetector, e.g. a photomultiplier tube. Preferably, the back-scatter signal so obtained is combined with a second signal representing, for example, either the level of forward-scatter within a prescribed, relatively narrow angular range, or the level of side-scattered radiation, or the level of attenuation of the cell-irradiating beam caused by the presence of the irradiated cell or particle in the beam, or the electrical impedance of the irradiated cell or particle, to differentiate the cells of interest. The method and apparatus of the invention are particularly useful in differentiating platelets and basophils in a blood sample.
REFERENCES:
patent: 3835315 (1974-09-01), Gravitt, Jr.
patent: 4702598 (1987-10-01), Bohmer
patent: 4882284 (1989-11-01), Kirchanski et al.
patent: 4914310 (1990-04-01), Jarofski
patent: 4939081 (1990-07-01), Figdor et al.
patent: 5017497 (1991-05-01), Gerard de Grooth et al.
patent: 5125737 (1992-06-01), Rodriguez et al.
patent: 5155044 (1992-10-01), Ledis et al.
patent: 5686308 (1997-11-01), Li et al.
patent: 6025201 (2000-02-01), Zelmanovic et al.
patent: 6137108 (2000-10-01), DeThomas et al.
patent: 6210969 (2001-04-01), Li et al.
patent: 6214625 (2001-04-01), Li et al.
patent: 6228652 (2001-05-01), Rodriguez et al.
patent: 6232125 (2001-05-01), Deka et al.
patent: 6743634 (2004-06-01), Kramer
patent: 02-207034 (2002-07-01), None
Eisert, W.G., “Cell Differentiation Based on Absorption and Scattering”,The Journal of Histochemistry and Cytochemistry, vol. 27, No. 1, pp. 404-409 (1979).
Sloot, et al., “Elastic Light Scattering from Nucleated Blood Cells: Rapid Numerical Analysis”,Applied Optics, vol. 25, No. 19, Oct. 1, 1986.
Sloot, et al., Scattering Matrix Elements of Biological Particles Measured in a Flow Through System: theory and Practice:,Applied Optics, vol. 28, No. 10, May 15, 1989.
Ault, K. A., “Flow Cytometric Measurement of Platelet Function and Reticulated Platelets”,Annals New York Academy of Sciences, 677:293-308 (1993).
Alter Mitchell E.
Coulter International Corp.
Kurz Warren W.
Wallenhorst Maureen M.
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