Apparatus for determining the presence or absence of MAI (Mycoba

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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435 72, 435 29, 435 32, 435 34, 435863, 435864, 435975, 4352871, 4352879, 4352883, C12M 100, C12M 114, C12M 116

Patent

active

054728770

ABSTRACT:
A method of speciating and identifying MAI in a specimen comprises placing a paraffin coated slide in a receptacle containing a sterile aqueous solution inoculated with the specimen, analyzing the slide after exposure to the specimen to determine the presence or absence of atypical Mycobacteria, and after the analysis step, if atypical Mycobacteria are determined to be present, performing at least one speciation assay to ascertain if the atypical Mycobacteria are MAI. A related apparatus is also disclosed for speciating and identifying MAI in a specimen comprising a paraffin-wax coated slide, a tube having a sterile aqueous solution contained therein, the tube adapted to hold the slide, and at least one speciation assay means for performing an assay to determine the presence or absence of MAI in the specimen after the specimen is introduced into the tube holding the solution and the slide. An apparatus and method for determining the sensitivity of MAI to different antibiotics and dosages thereof is also provided.

REFERENCES:
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Fuhs, G. W., "Der Mikrobielle Abbau Von Kohlenwasserstoffen", Arch. Mikrobiol., 39:374-422 (1961).
Mishra, S. K. et al., "Observations On Paraffin Baiting As A Laboratory Diagnostic Procedure In Nocardiosis", Mycopathologica and Mycologia Applicatia, vol. 51, 2-3, pp. 147-157 (1973).
Kirihara, J. M. et al., "Improved Detection Time For Mycobacterium avium Complex and Mycobacterium tuberculosis With BACTEC Radiometric System", J. Clin. Microbiol. 22:841-845 (1985).
Gonzalez, R., et al., "Evaluation of Gen-Probe DNA Hybridization Systems For The Identification Of Mycobacterium tuberculosis and Mycobacterium avium-intracellulare", Diagn. Microbiol. Infect. Dis., 8:69-77 (1987).
Wallace, J. M., et al., "Mycobacterium avium Complex Infection In Patients With Acquired Immunodeficiency Syndrome--A Clinicopathologic Study", Chest 93(5):926-932 (1988).

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