Apparatus for determining cell membrane dielectric breakdown

Details Apparatus for determining cell membrane dielectric breakdown Apparatus for determining cell membrane dielectric breakdown

1981-01-13

1983-01-11

Turk, Arnold

Electricity: measuring and testing

Conductor identification or location

Inaccessible

204195B, 324 71R, 422 68, 436 63, 436151, G01N 2704

Patent

active

043684238

ABSTRACT:
An apparatus which enables cell membrane dielectric breakdown to be determined. This determination is essential for the rapid inexpensive, reliable, in vitro quantification of lymphocyte tumor-cell cytotoxicity, lymphocyte membrane fluidity and the analysis of abnormal or tumor cells. The apparatus incorporates therein a cell dispenser used in conjunction with a DC electric field exposure tube. Electronic circuitry and its associated components process, acquire and store cell voltage signals as the cells are drawn through the electric field exposure tube. The procedure utilized with the apparatus of this invention involves quantifying the breakdown voltage required to electrically collapse the cell membrane. The electrical collapse is termed a dielectric breakdown and is characterized by a reversible drop in the cell's resistivity.

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