Apparatus for detecting denaturation of nucleic acid

Details Apparatus for detecting denaturation of nucleic acid Apparatus for detecting denaturation of nucleic acid

1994-07-15

1997-02-04

Warden, Jill

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing liquid or solid sample

422 681, 422 8205, 435 6, 435 912, 4352872, 4352887, 935 87, G01N 2164

Patent

active

055995047

ABSTRACT:
An apparatus for detecting denaturation of a nucleic acid, including: denaturation condition controlling element for controlling condition of denaturation under which a double-strand nucleic acid is separated into a first single-strand nucleic acid and a second single-strand nucleic acid; excitation light irradiation source for irradiating the double-strand nucleic acid before denaturation, and the first single-strand nucleic acid and second single-strand nucleic acid after the denaturation; fluorescence detection monitor for detecting fluorescence emission based on the excitation light irradiation; and processing element for receiving, storing and processing a signal supplied from the fluorescence detection monitor.

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patent: 5364759 (1994-11-01), Caskey et al.
patent: 5401847 (1995-03-01), Glazer et al.
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Morrison et al., Biochemistry, (1993), 32, 3095-3104.
Verpy, Proc. Nat. Acad. Sci, USA, 91, 1873-1877, (1994).
Freifelder, Physical Biochemistry: Applications to Biochemistry and Molecular Biology, 2nd Edition, W. H. Freeman and Comany (New York) pp. 19-20.

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