Apparatus for detecting cell chemotaxis

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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C435S288500, C435S288700, C435S285100, C435S285200, C435S285300, C436S063000

Reexamination Certificate

active

07807451

ABSTRACT:
The present invention relates to an improvement in an apparatus for detecting chemotaxis of cells. It aims at providing a structure for detecting chemotaxis of cells at an elevated accuracy with the use of a microquantity of cells. That is to say, an object of the present invention is to provide an apparatus for detecting chemotaxis of cells by which cell injection and position control can be easily carried out while ensuring the prevention of unexpected migration of the cells definitely positioned in a well or the injected sample so that a stable concentration gradient due to the diffusion of the specimen can be maintained and which ensures further automated operation and controlling.Namely, an apparatus for detecting chemotaxis of cells with a structure wherein two wells are connected to each other via a channel having resistance to the passage of cells and each well has an opening for injecting cells or a specimen, characterized by having (1) a means of transporting a liquid and a means of stopping the transportation after the injection or the aspiration discharge of the liquid and (2) a means of sealing the opening(s) in one or both of the cell-injection side and the specimen-injection side.

REFERENCES:
patent: 3888770 (1975-06-01), Avital et al.
patent: 3929583 (1975-12-01), Sharpe et al.
patent: 4317726 (1982-03-01), Shepel
patent: 4493815 (1985-01-01), Fernwood et al.
patent: 4514495 (1985-04-01), Schalkowsky et al.
patent: 4714674 (1987-12-01), Palladino
patent: 4729949 (1988-03-01), Weinreb et al.
patent: 4833382 (1989-05-01), Gibbs
patent: 4895805 (1990-01-01), Sato et al.
patent: 4912057 (1990-03-01), Guirguis et al.
patent: 5023054 (1991-06-01), Sato et al.
patent: 5284753 (1994-02-01), Goodwin, Jr.
patent: 5302515 (1994-04-01), Goodwin, Jr.
patent: 5744366 (1998-04-01), Kricka et al.
patent: 6238874 (2001-05-01), Jarnagin et al.
patent: 6395505 (2002-05-01), Goodwin, Jr.
patent: 2002/0009796 (2002-01-01), Goodwin, Jr.
patent: 2002/0086280 (2002-07-01), Lynes et al.
patent: 2003/0003570 (2003-01-01), Kanegasaki et al.
patent: 2003/0003571 (2003-01-01), Kanegasaki et al.
patent: 19917848 (2000-10-01), None
patent: 0368241 (1990-05-01), None
patent: 257366/1991 (1991-11-01), None
patent: 23967/1996 (1996-01-01), None
patent: 165062/1999 (1999-06-01), None
patent: 2002-159287 (2002-06-01), None
patent: WO-94/16098 (1994-07-01), None
patent: WO-96/03206 (1996-08-01), None
patent: WO 98/52691 (1998-11-01), None
patent: WO-98/52691 (1998-11-01), None
patent: WO-00/07007 (2000-02-01), None
patent: WO-01/32827 (2001-05-01), None
patent: WO 02/30486 (2002-04-01), None
patent: WO-02/46355 (2002-06-01), None
Kikuchi etal., Microchannel Array Flow Analyzer for measurement of Whole Blood Rheology and Flow Characteristics of Leukocytes Activated by Bacterial Stimulation, Feb. 1997, The International Society of Optical Engineering, vol. 2978, pp. 165-171.
Boyden, Department of Experimental Pathology, John Curtin School of Medical Research, Australian National University, Canberra, pp. 453-466 (1961).
Francis et al., Proc. Natl. Acad. Sci. USA, vol. 94, pp. 12258-12262 (Nov. 1997).
Kikuchi, Microvascular Research, vol. 50, pp. 288-300 (1995).
Kobayashi et al., J. Biochem, vol. 117, pp. 758-765 (1995).
Lehninger, Biochemistry, Second Edition, The Molecular Basis of Cell Structure and Function, pp. 173-181 (1978).
Mazumder et al., Journal of Crystal Growth, vol. 224, pp. 165-174 (2001).
Cutler, J. et al., Proc. Soc. Exp. Biol. Med., 147: 471-474 (1974).
John, T. J. et al., Life Science, 18:177-182 (1976).
Harvath, L. et al., Journal of Immunological Methods, 37:39-45 (1980).
Zigmond, S. H. et al., Annual Review of Medicine, 37:149-155 (1986).
Falk, W. et al., Journal of Immunological Methods, 33:239-247(1980).
Gatewood, B. et al., Journal of Immunology, 147:243-246(1991).
Falk, W. et al., Infection and Immunity; 36:450-454(1982).
Harvath, L. et al., Infection and Immunity, 36:443-449(1982).
Richards, K. K. et al., Immunological Communications, 13:49-62(1984).
Nelson, R. D. et al., J. Immunol., 115:1650-1656(1975).
Repo, H., Stand. J. Immunol., 6:203-209(1977).
Junger, W. G. et al., J. Immun. Methods, 160:73-79(1993).
Kikuchi, Y. et al., Microvascular Research, 44:226-240(1992).
Kikuchi, Y. et al., SPIE 2978:165-171(1997).
Kikuchi, Y. et al., Journal of the Japan Society for Precision Engineering, 62:1553-1556(1996).
Kikuchi, Y. et al., Chemical Engineering, 62:136-138(1998).
Kikuchy, Y. et al., Biophysics 37:254-258(1997).
Zigmond, S. H., J. Cell Biology 75:606-616(1977).
Allen, W. E. et al., J. Cell Biology, 141:1147-1157(1998).
Zicha, D. et al., J. Cell Science 99:769-775(1991).
Neuro Probe, Inc. “Neuro Probe Zigmond Chamber”.
Weber Scientific, Inc. “Dunn Chemotaxis Chamber”.
Fisher et al., J. Cell Biology, vol. 108, pp. 973-984 (1989).
Hayamizu et al., Sensors and Actuators A, vol. 103, pp. 83-87 (2003).
Supplementary European Search Report dated Jul. 6, 2009.

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