Apparatus for detecting back-scatter in a laser-based blood...

Optics: measuring and testing – By particle light scattering – With photocell detection

Reexamination Certificate

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Reexamination Certificate

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06894779

ABSTRACT:
A back-scatter detector for detecting light scattered backwardly (i.e., reflected) by an irradiated particle such as a blood cell comprises a plurality of optical fibers. A fiber optic holder having a centrally located opening for passing a light beam used to irradiate particles at a particle-interrogation zone serves to position the light-collecting ends at a desired position to collect back-scattered light. Preferably, the light-collecting ends of the optical fibers are positioned in a circular pattern centered about the irradiating light beam, and the respective axes of the supported portions of the optical fibers extend either parallel to the beam axis, or, more preferably, so that they converge at or near the location of the scattering source, i.e., the irradiated particle or cell. The latter configuration assures that the back-scatter light enters the fiber end substantially parallel to the fiber axis, thereby reducing optical transmission loses in the fiber.

REFERENCES:
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patent: 4702598 (1987-10-01), Bohmer
patent: 4914310 (1990-04-01), Jarofski
patent: 5461476 (1995-10-01), Fournier
patent: 6137108 (2000-10-01), DeThomas et al.
patent: 61-23947 (1986-01-01), None
Eisert, W.G., “Cell Differentiation Based on Absorption and Scattering”,The Journal of Histochemistry and Cytochemistry, vol. 27, No. 1, pp. 404-409 (1979).
Sloot, et al., “Elastic Light Scattering from Nucleated Blood Cells: Rapid Numerical Analysis”,Applied Optics, vol. 25, No. 19, Oct. 1, 1986.
Sloot, et al., Scattering Matrix Elements of Biological Particles Measured in a Flow Through System: theory and Practice:,Applied Optics, vol. 28, No. 10, May 15, 1989.

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