Apparatus for desorption and ionization of analytes

Details Apparatus for desorption and ionization of analytes Apparatus for desorption and ionization of analytes

2006-08-10

2009-02-17

Alexander, Lyle A (Department: 1797)

Chemistry: analytical and immunological testing

Including sample preparation

Liberation or purification of sample or separation of...

C436S174000, C436S173000, C436S063000, C436S155000, C250S287000

Reexamination Certificate

active

07491549

ABSTRACT:
This invention relates generally to methods and apparatus for desorption and ionization of analytes for the purpose of subsequent scientific analysis by such methods, for example, as mass spectrometry or biosensors. More specifically, this invention relates to the field of mass spectrometry, especially to the type of matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry used to analyze macromolecules, such as proteins or biomolecules. Most specifically, this invention relates to the sample probe geometry, sample probe composition, and sample probe surface chemistries that enable the selective capture and desorption of analytes, including intact macromolecules, directly from the probe surface into the gas (vapor) phase without added chemical matrix.

REFERENCES:
patent: 3896661 (1975-07-01), Parkhurst et al.
patent: 4022876 (1977-05-01), Anbar
patent: 4295046 (1981-10-01), Gruter et al.
patent: 4296332 (1981-10-01), Hill
patent: 4454233 (1984-06-01), Wang
patent: 4468468 (1984-08-01), Benninghoven et al.
patent: 4665192 (1987-05-01), Cerami
patent: 4686366 (1987-08-01), Stuke
patent: 4694167 (1987-09-01), Payne et al.
patent: 4705616 (1987-11-01), Andresen et al.
patent: 4902627 (1990-02-01), Kidwell
patent: 4988879 (1991-01-01), Zare et al.
patent: 5003059 (1991-03-01), Brennan
patent: 5045694 (1991-09-01), Beavis et al.
patent: 5073713 (1991-12-01), Smith et al.
patent: 5078135 (1992-01-01), Caprioli et al.
patent: 5118937 (1992-06-01), Hillenkamp et al.
patent: 5124267 (1992-06-01), Humpel et al.
patent: 5171989 (1992-12-01), Williams et al.
patent: 5209919 (1993-05-01), Turteltaub et al.
patent: 5260571 (1993-11-01), Cottrell et al.
patent: 5317932 (1994-06-01), Westlake, III et al.
patent: 5547835 (1996-08-01), Koster
patent: 5622824 (1997-04-01), Koster
patent: 5719060 (1998-02-01), Hutchens et al.
patent: 5894063 (1999-04-01), Hutchens et al.
patent: 6020208 (2000-02-01), Hutchens et al.
patent: 6027942 (2000-02-01), Hutchens et al.
patent: 6060237 (2000-05-01), Nygren et al.
patent: 6124137 (2000-09-01), Hutchens et al.
patent: 6225047 (2001-05-01), Hutchens et al.
patent: 6579719 (2003-06-01), Hutchens et al.
patent: 7074619 (2006-07-01), Hutchens et al.
patent: 7413909 (2008-08-01), Hutchens et al.
patent: 3221681 (1983-12-01), None
patent: 0 084 086 (1983-07-01), None
patent: 0 333 912 (1989-09-01), None
patent: 2235528 (1991-03-01), None
patent: 2235529 (1991-03-01), None
patent: 2236184 (1991-03-01), None
patent: 2236185 (1994-03-01), None
patent: WO 91/02961 (1991-03-01), None
patent: WO 92/13629 (1992-08-01), None
patent: WO 96/37777 (1996-11-01), None
patent: WO 96/40888 (1996-12-01), None
patent: WO 99/06833 (1999-02-01), None
American Biotechnology Laboratory, Feb. 1994 cover, cover-page 2 (1994).
Beavis, R. et al., “Epitaxial protein inclusion in sinapic acid crystals” J. Phys. D: Appl. Phys.26:442-447 (1993).
Brockman, A. et al., “Probe-immobilized affinity chromatography/mass spectrometry” Anal. Chem. 67(24):4581-5 (1995) [Abstract].
Brodbelt et al., “In vivo mass spectrometric determination of organic compounds in blood with a membrane probe” Anal. Chem. 59:454-458 (1987).
Dwyer, J. et al., “A novel sample preparation device for MALDI-MS” Intn'l Lab. 13A-13F (1997).
Fitzgerald, M. et al., “Direct characterization of solid phase resin-bound molecules by mass spectrometry” Bioorg. Med. Chem. Lett. 6(8):979-982 (1996) [Abstract].
Hillenkamp, F., “Laser desorption mass spectrometry: Mechanisms techniques and applications” Bordeaux Mass Spect. Conference Report 11A:354-362 (1988).
Hutchens, T. et al., “Differences in the confrontational state of a zinc-finger DNA-binding protein domain occupied by zinc and copper revealed by electrospray ionization mass spectrometry” Rapid Comm. in Mass Spect. 6:469-473 (1992).
Hutchens, T. et al., “New desorption strategies for the mass spectrometric analysis of macromolecules” Rapid Comm. in Mass Spect. 7:576-580 (1993).
Jonsson, G., et al., “Plasma desorption mass spectrometry of peptides and proteins adsorbed on nitrocellulose” Anal. Chem. 58(6):1084-1087 (1986).
Karas, M. et al., “Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons” Anal. Chem. 60:2299-2301 (1988).
Karas, M. et al., “Ultraviolet laser desorption of proteins up to 120,000 daltons” Bordeaux Mass Spect. Conference Report 11A:416-417 (1988).
Karas, M. et al., “UV laser desorption/ionization mass spectrometry of femtomol amounts of large proteins” Biomed. Environ. Mass Spectr. 18:841-843 (1989).
Mock, K. et al., “Sample Immobilization protocols for matrix-assisted laser desorption mass spectrometry” Rapid Comm. in Mass Spec. 6:233-238 (1994).
Nelson et al., “Mass spec. analysis of a trans-metal-binding peptide using MALDTOFMS: A demonstration of probe tip chemistry” Rapid Comm. Mass Spec. 6:4-8 (1992).
Nelson et al., “Mass spectrometric immunoassay” Anal. Chem. 67:1153-58 (1995).
Rivera, A., “A summary statement: Probes for affinity mass spectrometry of phophoproteins” Apr. 16, 1994.
Siuzdak, G. et al., “Applications of mass spectrometry in combinatorial chemistry” Biotech. Bioeng. 61(2):127-134 (1998) [Abstract].
Speir, J. et al., “Substrater-assisted laser desorption of neutral peptide molecules” Anal. Chem. 64:1041-1045 (1992).
Strupat, K. et al., “2,5-Dihydroxybenzoic acid: A new matrix for laser desorption-ionization mass spectrometry” Int'l J. Mass Spectr. and Ion Proc. 111:89-102 (1991).
Van Breemen et al., “Time-resolved laser desorption mass spectrometry. I. Desorption of performed ions” Interntnl. J. Mass Spectro. Ion Phys. 49: 36-51 (1983).
Xiang, F. et al., “A method to increase contaminant tolerance in protein matrix-assisted laser desorption/ionization by the fabrication of thin protein-doped polycrystalline films” Rapid Comm. in Mass Spec. 8:199-204 (1994).
Yip, T. et al., Protein Expression and Purification 2:355-362 (1991).

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