Chemistry: molecular biology and microbiology – Apparatus – Microorganism preservation – storage – or transport apparatus
Patent
1996-11-14
1998-07-14
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Microorganism preservation, storage, or transport apparatus
435260, 4352831, 62346, 62347, 34 62, C12M 102
Patent
active
057802955
ABSTRACT:
This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in an appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.
REFERENCES:
patent: 3228838 (1966-01-01), Rinfret
patent: 3309777 (1967-03-01), Hutton
patent: 3765189 (1973-10-01), Le Diouron
patent: 3892876 (1975-07-01), Hobday et al.
patent: 4329787 (1982-05-01), Newton
patent: 4559298 (1985-12-01), Fahy
patent: 4567847 (1986-02-01), Linner
patent: 4688387 (1987-08-01), Conaway
patent: 4707998 (1987-11-01), Linner et al.
patent: 4807442 (1989-02-01), Linner et al.
patent: 4865871 (1989-09-01), Livesey et al.
patent: 4874690 (1989-10-01), Goodrich, Jr.
patent: 4914927 (1990-04-01), Miller et al.
patent: 4964280 (1990-10-01), Piunno et al.
patent: 4980277 (1990-12-01), Junnilla
patent: 5024830 (1991-06-01), Linner et al.
patent: 5045446 (1991-09-01), Goodrich, Jr.
patent: 5153004 (1992-10-01), Goodrich, Jr.
patent: 5171661 (1992-12-01), Goodrich, Jr.
patent: 5178884 (1993-01-01), Goodrich
patent: 5269077 (1993-12-01), Bruttini
patent: 5292030 (1994-03-01), Kateman et al.
patent: 5336616 (1994-08-01), Livesey et al.
patent: 5364756 (1994-11-01), Livesey et al.
Meryman, H.T., "Absence of Unfrozen Freezable Water in Rapidly Frozen Red Cells," 7 Cryobiology, pp. 252-255 (19).
Terracio, L. and Schwabe, K.G., "Freezing and Drying of Biological Tissues for Electron Microscopy," 29 Journal of Histochemistry and Cytochemistry, pp. 1021-1028 (1981).
Coulter, H.D. and Terracio, L., "Preparation of Biological Tissue for Electron Microscopy by Freeze-Drying," 187 Anatomical Record, pp. 477-493 (1977).
Escaig, Jacques, "New Instruments Which Facilitate Rapid Freezing at 83K and 6K," Journal of Microscopy, Jun. 1982, pp. 221-222.
Boyne, Alan F., "A Gentle, Bounce-Free Assembly for Quick-Freezing Tissues for Electron Microscopy: Application to Isolated Torpedine Ray Electrocyte Stacks," Journal of Neuroscience Methods, 1, pp. 353-364 (1979).
Coulder, H. David, "Freezing and Drying of Biological Tissues With a Toggle-Link Helium Freezer and an Improved Freeze-Drying Apparatus: Application to Neuropeptide Immunocytochemistry," Journal of Electron Microscopy Technique, 4, pp. 315-328 (1986).
Reichert-Jung, "Cryovacublock"--Instruction Manual ( No date provided).
Escaig, Jacques, "Control of Different Parameters for Optimal Freezing Conditions," Science of Biological Specimen Preparation, pp. 117-122 ( No date provided).
Handley, Dean A., Alexander, Jack T., and Chien, Shu, "The Design and Use of a Simple Device for Rapid Quench-Freezing of Biological Samples," Journal of Microscopy, Mar. 1981, pp. 273-282, Polaron Instruments, Inc., The Slammer, (brochure).
Med-Vac, Inc., "Cryopress" (Brochure) ( No date provided).
Reichert-Jung, "Cryofrace 190 Cryoblock" (Brochure) ( No date provided).
Quick Freezing Devices, "Quick Freezing by Bounce-Free Delivery" (Brochure) (No Date provided).
Moor, H., Bellin, G., Sandri, C. and Akert, K., "The Influence of High Pressure Freezing on Mamalian Nerve Tissue," Cell and Tissue Research, pp. 201-216 (1980).
Lejeune et al., Ann Pharm. Fr., 44(6), pp. 461-466 (1986).
Hayakawa, K., Hakkokogaku Kaishi, 63(1), pp. 17-22 (1985).
Roinel, N., . Electron Microsc. Tech., 9(1), pp. 45-46 May 1988.
Mayer, "New method for vitrifying water and other liquids by rapid cooling of their aerosols" Jul. 15, 1985 J. Appl. Phys. 58(2) Jul. 1985 pp.663-667 .
del Campo Anthony A.
Livesey Stephen A.
Nag Abhijit
Nichols Ken B.
Piunno Carmen
Beisner William H.
Life Cell Corporation
LandOfFree
Apparatus for cryopreparation, dry stabilization and rehydration does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Apparatus for cryopreparation, dry stabilization and rehydration, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus for cryopreparation, dry stabilization and rehydration will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1880908