Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1979-07-31
1981-04-21
Castel, Benoit
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435808, 422 81, C12M 134
Patent
active
042634067
ABSTRACT:
A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.
REFERENCES:
patent: 3933430 (1976-01-01), Hare
Schroeder; R. R. et al.; "Enzyme-Selective Detector Systems for High Pressure Liquid Chromatography", Jour. of Chromatography; vol. 134, pp. 83-90, (1977).
Schlabach; T. D. et al.; "A Continuous-Flow Enzyme Detector for Liquid Chromatography"; Journal of Chromatography; vol. 134, pp. 91-106, (1977).
Bostick William D.
Denton Mark S.
Dinsmore Stanley R.
Carter C. Clay
Castel Benoit
Hamel Stephen D.
The United States of America as represented by the United States
Uzzell Allen H.
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