Apparatus for contacting biological cell systems with a perfusio

Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus

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435285, 435310, 435 1, 417437, C12M 300, C12M 304, C12M 114

Patent

active

047627948

ABSTRACT:
Apparatus for contacting a biological cell system on a substrate with a pulsating flow of perfusion fluid has a two-part housing for a combined holding and tensioning device which is removably inserted into an internal space to define with the housing and with the substrate therein two separate chambers. The housing has inlets and outlets for the two chambers and inlet and outlet valves which are operated in response to a reduction or increase of the volume of the respective chambers by elastic walls of the housing. The walls are oscillatable by cams and cam followers and the valves at the inlets open when the valves at the respective outlets are closed and vice versa. The characteristics of perfusion fluid prior to entry into and upon evacuation from the chambers are monitored to thus ascertain the nature of processes which take place at the cells on the substrate.

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