Coating apparatus – With indicating – testing – inspecting – or measuring means – With means for visual observation
Patent
1985-05-23
1986-12-02
Lawrence, Evan K.
Coating apparatus
With indicating, testing, inspecting, or measuring means
With means for visual observation
65284, 118 66, 118643, 350244, B05C 1100
Patent
active
046256776
ABSTRACT:
An apparatus for coating and polishing a micropipette for patch recording comprises an inverse microscope having a stage for supporting a micropipette to be processed, and a pair of objectives of relatively low and relatively high magnification, respectively, which are adapted to be selectively placed into the optical path of the microscope. An air gun is positioned to supply an air jet to a pipette supported on the stage, said gun comprising heating means for selectively producing a hot and a cold air jet. An attchment comprising an electricially heatable filament is associated to the objective of relatively high magnification. The micropipette is positioned and coated under observation with the aid of the objective of low magnification and then the coating is cured by applying the hot air jet. Subsequently, the tip of the pipette is polished by the heated filament under observation with the aid of the objective of high magnification. These steps may be performed without the necessity of changing or readjusting the position of the micropipette between these two processing steps.
REFERENCES:
patent: 4262426 (1981-04-01), Miyazaki
patent: 4510442 (1985-04-01), Neher
Hawley, G. G., "The Condensed Chemical Dictionary", Ninth Edition, New Yo Van Nostrand Reinhold Company, 1977, pp. 832, 949.
Hamill, O. P. et al., "Improved Patch-Clamp Techniques . . . Patches", Pflugers Archiv-European Journal of Physiology, 391 (1981), p.p. 85-100.
Corey, D. P. and Stevens, C. F., "Science and Technology of Patch-Recording Electrodes", In: Sakmann, B. and Neher, E. (eds), Single Channel Recording (New York, Plenum Press, 1983), pp. 53-68.
Lawrence Evan K.
Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
Nields Henry C.
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