Apparatus for capillary electrophoresis and associated method

Details Apparatus for capillary electrophoresis and associated method Apparatus for capillary electrophoresis and associated method

2005-01-11

2005-01-11

Gakh, Yelena G. (Department: 1743)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S454000, C204S600000, C204S601000

Reexamination Certificate

active

06841054

ABSTRACT:
An apparatus and method for capillary zone electrophoresis includes a polyelectrolyte multilayer positioned in a capillary tube for analytical separations of macromolecules. The capillary comprises a passage defined by passage walls comprising fused silica. The polyelectrolyte multilayer is positioned within the passage adjacent the walls, and comprises an organic polyelectrolyte. The passage may further comprise nonporous silica particles coated with a multilayer including a plurality of polyelectrolyte layers. An apparatus includes a power supply having a positive electrode and a negative electrode for generating an electric field therebetween. The apparatus includes a capillary having a passage formed by passage walls and comprising therein a polyelectrolyte multilayer positioned substantially within the passage. The passage has a first end electrically connected to the positive electrode and a second end electrically connected to the negative electrode to thereby generate an electric field through the passage. The apparatus also includes a sensor positioned adjacent the passage for sensing macromolecules.

REFERENCES:
Katayama et al. “Stable cationic capillary coating with successive multiple ionic polymer layers for capillary electrophoresis”, Anal. Chem., 1998, v. 70, pp. 5272-5277.*
Zhao et al. Anal. Chem., 1993, 65, 2747-2752.

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