Apparatus for blot screening numerous, small volume, antibody so

Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition

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435293, 435301, C12M 112, C12M 120, G01N 3348

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active

048349461

ABSTRACT:
A cover plate, having a lower, flat surface containing an array of numerous, parallel, closely spaced apart, downwardly opening channels, is releasably clamped upon a flat support plate surface. The channels each have inlet and outlet holes communicating with the upper surface of the cover plate. A paper-like, resilient cushion sheet is clamped between the plate surfaces and a paper-like membrane, carrying a pattern of antigen-like material, is arranged between the sheet and the cover plate channeled surface. The channels are very narrow, shallow and many times longer than their widths and are closed by the stripe-like portions of the membrane which overlie the channels. The cushion sheet is compressed between adjacent channels and bulges upwardly at each of the channels to thereby bulge the membrane stripe-like portions into dome-shaped cross-sections extending into the channels. This reduces the volumes of the channels and increases the surface areas of the membrane stripe-like portions that are exposed within the channels. Numerous drop-size samples of antibody-type solutions are inserted into the channels through channel inlet holes, that extend through the cover plate, for reacting with the material carried on the stripe-like portions of the membrane. A removable manifold having a pair of elongated grooves may be temporarily applied upon the cover plate so that the grooves overlie all of the channel inlet holes and all of the channel outlet holes for rapidly, simultaneously filling and draining all the channels.

REFERENCES:
patent: 4493815 (1985-01-01), Fernwood
patent: 4526690 (1985-07-01), Kiovsky et al.
patent: 4591567 (1986-05-01), Britten et al.
patent: 4681853 (1987-07-01), Hardy et al.
patent: 4713349 (1987-12-01), Levin
Turbo Blot.TM. brochure by American Bio Nuclear.
Flyer, "Immunetics", (approximately June/July, 1986), 1 page-front and back.

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