Apparatus for analysis of physiological fluids

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Sample mechanical transport means in or for automated...

Reexamination Certificate

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Details

C422S105000, C422S105000, C436S054000, C436S180000

Reexamination Certificate

active

06689318

ABSTRACT:

The present invention relates to automatical introduction of reference fluid in apparatus for analysis of physiological fluids.
A known apparatus of this type is an apparatus for blood gas analysis. Such apparatus are by way of example used in hospitals and medical laboratories where they are permanently ready for operation with a view to analysis of blood samples immediately after their being taken. The apparatus analyses the blood samples for example by measuring partial pressure of CO
2
(pCO
2
), partial pressure of O
2
(pO
2
) and pH. In order that an apparatus works in a reliable way, i.e. provides reliable measuring results, it must normally be calibrated at appropriate intervals. Furthermore, the calibration must be controlled frequently. Therefore, a quality control procedure is, among other, performed where the apparatus analyses a control solution with known values for at least some of the parameters which the apparatus is adapted to measure. According to guidelines from National Committee for Clinical Laboratory Standards (NCCLS DOCUMENT C27-A, Vol. 13, No. 6) for collection and handling an arterial specimen for pH and blood gas analysis and for calibrating the analyzer together with recommendations for an acceptable quality control program, it is recommended to perform said quality control program once per shift (i.e. three times a day by treble shift) such that three different levels (high, middle and low) for the parameters to be controlled are measured in three consecutive shifts.
So far this quality control has been performed manually in the way that an operator injects or introduces a control solution with specific contents in the analysis apparatus according to a fixed schedule, and the apparatus then carries out a control program. The result of this control is printed and if the measured values of the parameters in question lie within predetermined limits, the control is accepted, and the apparatus may be used. Otherwise it may be necessary to calibrate the apparatus before it is used for analysis of a sample. The control program is terminated by a rinse program by which the apparatus rinses itself of control solution.
The control solution is usually supplied in small containers or ampoules, the contents of one ampoule being used for one quality control. The ampoules are usually designed as ampoules with a breakable neck. Larger containers for several controls are also known. The containers for control solutions are described in the literature since the beginning of the 1970s. One of the latest publications on containers with control solutions is U.S. Pat. No. 5,230,427.
The ampoule contains both a liquid phase and a gas phase, a temperature dependent balance being between dissolved gas in the liquid phase and gas in the gas phase. In order to know the exact contents of a given gas in the solution, it is therefore necessary that the temperature of the solution in the ampoule is known, and that this temperature is uniform within the entire volume of the ampoule. In order to ensure a uniform temperature distribution, the ampoule is shaked prior to the quality control. Alternatively, it may be ensured that the ampoule has been kept at a constant known temperature for some time.
A problem with the prior art technology of manual quality control is that in the nature of the case, it requires qualified personnel efforts as there are some (human) error possibilities, for instance that a quality control is forgotten or that the quality control procedure is carried through incorrectly.
It is the object of the invention to avoid or reduce the drawbacks of the prior art.
According to the invention, an apparatus for analysis of physiological fluids, such as an apparatus for blood gas analysis, is characterized by equipment for automatic introduction of reference fluid for the apparatus, which equipment comprises a holder for concurrent hold of several sealed containers with reference fluid, means for bringing a selected container and means for opening the container into a specific position in relation to each other, means for bringing the opened container and an inlet aggregate for introduction of fluid from the container into the apparatus into a specific position in relation to each other, means for activating the inlet aggregate, means for removing the container and the inlet aggregate from each other, and programmable control means for control of the introduction of reference fluid into the apparatus.
The holder may be a detachable or non-detachable part of the apparatus. Further, the holder may comprise a device into which the sealed containers may be loaded individually or it may comprise a support means adapted for removably holding or being attached to a magazine wherein several sealed containers are concurrently held.
The control means of the device then ensures the introduction of reference fluid at pre-programmed times, the control means selecting a container with a specific reference fluid and the device then automatically ensures that the container is opened, its contents introduced into the apparatus and the container and the inlet aggregate removed from each other.
In a preferred embodiment, the inlet aggregate is constituted by the inlet aggregate of the apparatus for fluid samples for analysis. Thereby is avoided a separate aggregate for transmission of the fluid from the container to the inlet aggregate of the apparatus for fluid samples to be analysed, such as blood samples.
The apparatus according to the invention may be designed in a particularly simple way if the means for bringing a selected container and the inlet aggregate into a specific position in relation each other comprise means for moving a magazine and/or the holder of the sealed containers. The means for moving the magazine and/or the holder may further be adapted to agitate the containers such that a shaking effect is obtained.
The means for bringing a selected container and the means for opening the container into a specific position in relation to each other and the means for bringing the opened container and an inlet aggregate for introduction of fluid from the container into the apparatus into a specific position in relation to each other may be constituted by the same means. Furthermore, said positions of the container in relation to means for opening the container and in relation to an inlet aggregate for introduction of fluid from the container into the apparatus may be one and the same position.
Preferably, the means for opening the container comprise an element which is made to press against an opening area on the container which is thus opened.
In all aspects of the invention, the sealed containers with reference fluid may or may not contain both a liquid phase and a gas phase, two phases being preferred.
According to the invention, there is further provided a unit to be mounted into and to be a part of an apparatus for analysis of physiological fluids, which unit is characterized in a holder for concurrent hold of several sealed containers with reference fluid, means for bringing a selected container and means for opening the container into a specific position in relation to each other, means for bringing the opened container and an inlet aggregate for introduction of fluid from the container into the apparatus into a specific position in relation to each other, and means for removing the container and the inlet aggregate from each other. When mounted, this unit forms an integrated part of an apparatus according to the invention as described above.
According to the invention, there is further provided an ampoule containing a reference fluid and comprising a body with an opening area having an opening the circumferential edge of which lies in one and the same plane, said body being hermetically sealed by a membrane mounted to the circumferential opening edge of the body, said ampoule being characteristic in that the membrane is planar and comprises a layer of a glass material. The membrane may consist of one or several layers of glass or the membrane may comprise at least o

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