Apparatus, for amplification of nucleic acids

Details Apparatus, for amplification of nucleic acids Apparatus, for amplification of nucleic acids

1988-12-16

1992-07-28

Housel, James C.

Chemical apparatus and process disinfecting, deodorizing, preser

Chemical reactor

Organic polymerization

422189, 422203, 422208, 435290, 435316, 935 88, C08F 300, B10J 800, C12M 138

Patent

active

051339404

ABSTRACT:
Amplification of nucleic acids is performed by incubating in a polymerization vessel a reaction mixture which contains in a suitable buffer solution, one or several single-stranded target nucleic acids, suitable primers, deoxyribouncleoside triphosphates and a polymerase. After a sufficient time for polymerication to occur, the reaction mixture is transferred into another vessel for the denaturation of the nucleic acids into single stranded nucleic acids. After denaturation, the reaction mixture is transferred back into the original vessel. The amplification process is regulated to maintain a temperature advantageous for the action of the polymerization enzyme in the polymerization vessel and a temperature advantageous for denaaturation in the denaturation vessel. Furthermore, the apparatus includes a liquid transfer system for transferring the reaction liquid from one vessel to another, which comprises at least one liquid transfer tube per vessel pair which extends from within the polymerization vessel to within the denaturation vessel.

REFERENCES:
patent: 4171427 (1979-10-01), Ohorodnik et al.
patent: 4478814 (1984-10-01), Kesten et al.
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683195 (1987-07-01), Mullis
patent: 4683202 (1987-07-01), Mullis
patent: 4753787 (1988-06-01), Krijgsman
patent: 4889812 (1989-12-01), Guinn et al.
Nucleic Acids Research vol. 16, No. 7, 1988 A Simple and Low Cost DNA Amplifier by Rolio et al., pp. 3105 and 3106.
DNA vol. 7, No. 6, 1988, pp. 441-447 Laboratory Methods-A Programmable System to Perform the Polymerase Chain Reaction by Heinz Ulrich and Joe W. Gray.
Polymerase chain reaction automated at low cost by N. S. Foulkes et al., pp. 5687-5688, Submitted May 17, 1988.

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