Apparatus containing a septum which impedes cell permeation for

Details Apparatus containing a septum which impedes cell permeation for Apparatus containing a septum which impedes cell permeation for

1990-04-05

1992-09-29

Johnston, Jill A.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435284, 435286, 435311, 435813, C12N 502, C12M 302, C12M 112

Patent

active

051513625

ABSTRACT:
By culturing cells in an apparatus containing a first zone in which a cell suspended medium is present and a second zone in which liquid containing a physiological saline solution and/or nutrient sources is present, the first zone being separated from the second zone by a septum with characteristics, such as pores size or electrostatic charges, which impedes the cells from freely permeating through, while allowing at least one of nutrient sources, cell wastes or the product to move between the first zone and the second zone via the septum to proliferate cells in the first zone, wherein a flow rate via the septum is controlled in such a manner that the cell count moved from the first zone into the second zone via the septum does not exceed the cell count increased by cell growth in the first zone. Thus, cell culture at a high cell density can be achieved in a large scale.

REFERENCES:
patent: 3743582 (1973-07-01), Atsuo et al.
patent: 4016044 (1977-04-01), Fresnel et al.
patent: 4218538 (1980-08-01), Church
patent: 4251633 (1981-02-01), Orlowski et al.
patent: 4440638 (1984-04-01), Judy et al.
patent: 4535062 (1985-08-01), Muller
patent: 4568643 (1986-02-01), Levy
patent: 4861725 (1989-08-01), Liau
Katsuta et al., In Vitro, 15(12) pp. 949-956 (1979).

Affiliated with

Also associated with

Rating

Apparatus containing a septum which impedes cell permeation for does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Apparatus containing a septum which impedes cell permeation for , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus containing a septum which impedes cell permeation for will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1968051