Apparatus and process for the detection and counting of rarely o

Details Apparatus and process for the detection and counting of rarely o Apparatus and process for the detection and counting of rarely o

1995-06-21

1998-05-12

Mancuso, Joseph

Image analysis

Applications

Biomedical applications

2504612, 356 39, 377 10, G06K 900, G06K 946

Patent

active

057518394

ABSTRACT:
Apparatus and process capable of rapid detection and counting of rarely occuring mammalian cells in blood and other tissues which have been labeled with a fluorescent dye.
The process includes: scanning a solid support on which a specimen potentially containing fluorescent cells has been deposited, with an incident beam from a laser, forming a laser spot on the solid support, the laser spot being substantially greater than the cells to be detected, the laser spot size being between 15 and 30 .mu.m; and simultaneously: detecting the resultant fluorescent light at least at one wavelength; establishing a set of correlated-features by a line-to-line correlation of individual features; comparing the correlated-features on each pair of adjacent lines in time synchrony, at least at two different wavelengths .lambda..sub.1 and .lambda..sub.2 ; making a size discrimination of retained events and selecting those having a size corresponding to the type of cells searched; determining if, for retained events after size discrimination, the events energy profile in three dimensions is within predetermined Gaussian shape criteria; and counting the remaining events to determine and to count exclusively the fluorescent cells present on the solid support.

REFERENCES:
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patent: 5523207 (1996-06-01), Kamentsky et al.
patent: 5578832 (1996-11-01), Trulson et al.
High-Speed Video Imaging and Digital Analysis of Microscopic Features in Contracting Striated Muscle Cells, Kenneth Roos and Stuart Taylor, Optical Engineering, Feb. 1993, vol. 32 No. 2 pp. 306-313.

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