Apparatus and process for continuous in vitro synthesis of prote

Details Apparatus and process for continuous in vitro synthesis of prote Apparatus and process for continuous in vitro synthesis of prote

1993-02-12

1995-07-18

Housel, James C.

Chemistry: molecular biology and microbiology

Miscellaneous

435291, 21032175, 935 88, B01D 6308

Patent

active

054340799

ABSTRACT:
An apparatus and process for continuous, cell-free, in vitro synthesis of peptides, particularly peptide-MHC complexes make use of a novel bioreactor flow cell which allows for the reproducible, systematic variation of single parameters in order to optimize translation processes. The bioreactor flow cell includes a pair of substantially parallel membranes positioned within a chamber to define therebetween a space, the ratio of total membrane surface area to chamber volume being at least 5 units.sup.-1, which permits high perfusion rates through the system with lower flux rate per membrane area. The apparatus also may include a novel countercurrent flow dialysis cell and other components.

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