Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1989-08-09
1991-08-20
Niebling, John F.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
2041828, G01N 2726, B01D 5702
Patent
active
050412035
ABSTRACT:
An apparatus and a procedure for fractionating DNA using agarose gel electrophoresis is provided. To improve resolution by length and conformation, the direction of the electric field impressed upon the sample is changed by rotating the gel. During agarose gel electrophoresis in one mode, a commerically available stepping motor and indexer are used to periodically re-orient to two different positions a gel on a circular disk within a conventional, horizontal, submerged gel electrophoresis apparatus. Because of the homogeneous field used, DNA forms comparatively sharp, undistorted bands. In one of several other modes, involving electrophoresis during periods of both continuous gel motion and stationary gel, the gel is rotated through integer multiples of 360.degree. followed by stationary electrophoresis.
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Support for the development of the present invention was received from the National Institutes of Health (grants GM24365 and AI22568).
Niebling John F.
Starsiak Jr. John S.
The University of Texas System
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