Apparatus and methods for sequencing nucleic acids in microfluid

Details Apparatus and methods for sequencing nucleic acids in microfluid Apparatus and methods for sequencing nucleic acids in microfluid

1997-10-16

1999-09-28

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 216 33, 356344, C12Q 168

Patent

active

059586940

ABSTRACT:
The present invention is generally directed to microfluidic systems and methods of using such systems in the determination of the nucleotide sequence of target nucleic acid sequences (referred to herein as the "target"). In particular, the present invention provides methods and systems for determining the relative positions within a target nucleic acid sequence that are occupied by a given nucleotide, e.g., A, T, G or C, by separating mixtures of nested sets of fragments of the target nucleic acid, which sets each include fragments that terminate in a different given nucleotide.

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