Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1994-06-22
1997-10-14
Chin, Christopher L.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
356318, 356317, 356244, 356246, 385 12, 385 34, 385129, 422 55, 422 57, 422 58, 422 8205, 422 8208, 422 8209, 422 8211, 4352871, 4352872, 4352887, 435808, 436164, 436165, 436172, 436527, 436805, 436807, 436809, G01N 33543, G01N 33552
Patent
active
056771967
ABSTRACT:
Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.
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Caldwell Karin D.
Christensen Douglas A.
Herron James N.
Huang Shao-Chie
Janatova Vera
Chin Christopher L.
University of Utah Research Foundation
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