Apparatus and methods for multi-analyte homogeneous...

Details Apparatus and methods for multi-analyte homogeneous... Apparatus and methods for multi-analyte homogeneous...

2005-12-27

2005-12-27

Chin, Christopher L. (Department: 1641)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C422S051000, C422S051000, C422S051000, C422S082050, C422S082080, C422S082110, C435S287200, C435S287900, C435S288500, C435S288700, C435S808000, C436S164000, C436S165000, C436S514000, C436S518000, C436S527000, C436S531000, C436S805000, C436S807000, C385S012000, C385S129000, C385S130000, C356S418000

Reexamination Certificate

active

06979567

ABSTRACT:
Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semicylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method has patches of capture molecules, each specific for a different analyte disposed adjacently within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers, which in turn are coupled to the waveguide surface or to a nonspecific binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers, the selective irradiation involving a mask, a laser light source, or the like.

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