Apparatus and methods for isoelectric focusing of amphoteric sub

Details Apparatus and methods for isoelectric focusing of amphoteric sub Apparatus and methods for isoelectric focusing of amphoteric sub

1990-05-18

1992-11-03

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041832, 204299R, 204301, G01N 2726

Patent

active

051605945

ABSTRACT:
The present invention includes novel isoelectric focusing cells and methods for isoelectric focusing of amphoteric substances within fluids containing carrier ampholytes which inhibit the phenomena known as spiking or acid notching. The apparatus includes a barrier separating the electrode compartments from the focusing cell compartments which minimizes hydrogen and hydroxyl ions contained in electrolyte solution within the electrode chambers from passing into the focusing cell passageways. The currently preferred barrier is a bipolar membrane; however, the combination of anion and cation selective membranes is also effective. Such a barrier enables very narrow pH gradients to be established and maintained, thereby permitting high resolution separations of amphoteric biological substances.

REFERENCES:
patent: 2878178 (1959-03-01), Bier
patent: 3079318 (1963-02-01), Bier
patent: 3657106 (1972-04-01), Smith
patent: 3677923 (1972-07-01), Bier
patent: 3686089 (1972-08-01), Korngold et al.
patent: 3751356 (1973-08-01), Takeya et al.
patent: 3788959 (1974-01-01), Smith
patent: 3901780 (1975-08-01), Denckla
patent: 3915839 (1975-10-01), Rilbe et al.
patent: 3919065 (1975-11-01), Heden
patent: 3951777 (1976-04-01), Denckla
patent: 3962058 (1976-06-01), Denckla
patent: 3972791 (1976-08-01), Stern
patent: 3989613 (1976-11-01), Gritzner
patent: 4040940 (1977-08-01), Bier
patent: 4043896 (1977-08-01), Ahlgren
patent: 4088561 (1978-05-01), Anderson
patent: 4130470 (1978-12-01), Rosengren et al.
patent: 4217193 (1980-08-01), Rilbe
patent: 4234404 (1980-11-01), Satoh
patent: 4243507 (1981-01-01), Martin et al.
patent: 4289596 (1981-09-01), Satoh
patent: 4362612 (1982-12-01), Bier
patent: 4385974 (1983-05-01), Shevitz
patent: 4396477 (1983-08-01), Jain
patent: 4401538 (1983-08-01), Hausfeld
patent: 4416761 (1983-11-01), Brown et al.
patent: 4441978 (1984-04-01), Jain
patent: 4484141 (1984-11-01), Cook et al.
patent: 4495279 (1985-01-01), Kapetsky et al.
patent: 4533447 (1985-08-01), Meldon
patent: 4588492 (1986-05-01), Bier
patent: 4670119 (1987-06-01), Hurd
patent: 4673483 (1987-06-01), Mandle
patent: 4963236 (1990-10-01), Rodkey et al.
Bier, M. "Forced-Flow Electrophoresis and its Biomedical Applications", Membrane Process for Industry--Proceedings of the Symposium (May 19-20, 1966), pp. 218-233.
Bier, M. et al., "Developments in Isoelectric Focusing," Peptides--Structure and Biological Function, Proceedings of Sixth American Peptide Symposium, 1979, pp. 79-89.
Fawcett, John S., "Continuous-Flow Isoelectric Focusing and Isotachophoresis, " Annals New York Academy of Sciences, pp. 112-126.
Fawcett, John S., "Continuous-Flow Isoelectric Focusing," Isoelectric Focusing, N. Catsimpoolas ed., Chapter 7 (1976), pp. 173-208.
Kolin, Alexander, "Isoelectric Focusing", Isoelectric Focusing, N. Catsimpoolas ed., Chapter 1 (1976), pp. 1-10.
Martin, A. J. P., et al., "New Apparatus for Isoelectrtic Focusing," Journal of Chromatography, vol. 159 (1978), pp. 101-110.
Nagabhushan, T. L. et al., "Application of recycling isoelectric focusing for purification of recombinant human leukocyte interferons," Electrophoresis 7 (1986), pp. 552-557.
Righetti, P. G. et al., "Theory and Fundamental Aspects of Isoelectric Focusing," Isoelectric Focusing, Chapter 1 (1976), pp. 341-376.
Rilbe, Harry, "Theoretical Aspects of Steady-State Isoelectric Focusing," Isoelectric Focusing, N. Catsimpoolas ed., Chapter 2 (1976), pp. 13-52.
Vesterberg, Olof, "The Carrier Ampholytes," Isoelectric Focusing, N. Catsimpoolas ed., Chapter 3 (1976), pp. 53-76.

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