Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2000-10-12
2002-12-03
Jones, W. Gary (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S283100, C435S287200, C435S288400, C422S068100, C536S023100
Reexamination Certificate
active
06489111
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to a reaction apparatus which can be used for a variety of reactions by heating a small amount of solution. Particularly, the present invention relates to a method for immobilized DNA library preparation, a method for gene amplification, a method for comparing genes systematically, an apparatus therefor and immobilized DNA library therefore.
BACKGROUND OF THE INVENTION
Conventionally, a polymerase-chain-reaction (PCR) method has been broadly utilized as means for amplifying genes. In the PCR method, a tube-shaped plastic reaction container which is filled with reaction solution is inserted into an aluminium block and so on with a cap for preventing the reaction solution from evaporating. The temperature of the aluminium block is periodically changed to a thermal metamorphic temperature, an annealing temperature and a DNA synthetic temperature in order. Such a thermal control cycle is repeated for a predetermined number of times.
However, in the conventional PCR method, the device used is a plastic reaction container which has relatively high thermal capacity and relatively low thermal conductivity. Further, the thermal control is operated with respect to an aluminium block itself. Therefore, a long time is required for heating/cooling. A target duplicated amount of DNA is relatively low, since thermal control of the reaction solution is not operated completely.
A purpose of the present invention is to resolve the above drawbacks and to provide an apparatus for gene amplification having simple operation wherein a step of heating/cooling can be operated rapidly and a thermal cycle of the reaction can be shortened. Another object of the present invention is to provide a method for amplifying genes.
Another purpose of the present invention is to provide a method for comparing genes systematically using the above apparatus.
SUMMARY OF THE INVENTION
An apparatus for preparing immobilized DNA library according to the present invention is an apparatus comprising a reaction body on which a groove portion for receiving a container is provided and a cap portion provided at an upper portion of the reaction body with means for heating/cooling and means for cooling, wherein the apparatus is characterized in that the container is made of at least one substrate which has been chemically modified.
A gene amplifying apparatus according to the present invention is an apparatus comprising a reaction body on which a groove portion for receiving a container is formed. A cap portion is provided at an upper portion of the reaction body with means for heating/cooling and means for cooling, wherein the apparatus is characterized in that the container is made of at least one substrate for immobilizing genes. In these apparatus, it is preferable that the substrates are solid-state substrates and the container is a separable type and a cassette type.
In a method for temperature control by the apparatus for amplifying genes according to the present invention, the method is characterized that a signal from a temperature measuring portion
61
is input to a computer control portion
64
and the computer control portion
64
compares the information input the signals and program chart previously input so that a thermal control portion
62
of means for heating/cooling
51
and a thermal control portion
63
of means for cooling
52
can be driven.
In a method for temperature control by the apparatus for amplifying genes according to the present invention, the method is characterized that a signal from a temperature measuring portion
61
is input to a computer control portion
64
and the computer control portion
64
compares with the signals and program chart previously input so that a thermal control portion
62
of means for heating/cooling
51
and a thermal control portion
63
of means for cooling
52
can be driven.
The substrates for immobilizing DNA according to the present invention are characterized in that the substrates are utilized in the above apparatuses.
A method for preserving the substrates in a refrigerator according to the present invention is characterized in that the substrates for immobilizing DNA is preserved in the refrigerator.
A method for comparing genes systematically according to the present invention is characterized in that a plurality of DNA-immobilized libraries are set in a container.
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patent: 5605662 (1997-02-01), Heller et al.
patent: 5656493 (1997-08-01), Mullis et al.
patent: 5777372 (1998-07-01), Kobashi
patent: 6004513 (1999-12-01), Albagli et al.
patent: 6043080 (2000-03-01), Lipshutz et al.
patent: 6210882 (2001-04-01), Landers et al.
Shoffner et al. “Chip PCR. 1. Surface passivation of microfabricated silicon-glass chips for PCR” Nucleic Acid Research, 1996, 24(2):375-379.*
Lamture, J. et al. “Direct Detection of Nucleic Acid Hybridization On the Surface of a Charge Coupled Device,” Nucleic Acids Research (1994), vol. 22, No. 11, p. 2121-2125.
Takahashi Kojiro
Tanga Michifumi
Browdy and Neimark , P.L.L.C.
Forman B J
Jones W. Gary
Toyo Kohan Co. Ltd.
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