Apparatus and methods for detecting DNA in biological samples

Details Apparatus and methods for detecting DNA in biological samples Apparatus and methods for detecting DNA in biological samples

2006-12-26

2006-12-26

Navarro, Mark (Department: 1645)

Chemistry: molecular biology and microbiology

Kit

Reexamination Certificate

active

07153687

ABSTRACT:
Apparatus and methods are described for detecting target DNA in a biological sample using capture probes and electrically-assisted hybridization. The reaction cell is formed with an attachment surface of aluminum oxide for better thermal and physical properties, and the aluminum oxide surface is coated with anti-DIG antibody to provide a convenient attachment layer for the capture probes allowing their correct orientation, while the capture probes are formed with a DIG-label so that they attach to the surface of the cell through an anti-DIG/DIG linkage.

REFERENCES:
patent: 5128247 (1992-07-01), Koller
patent: 5632957 (1997-05-01), Heller et al.
patent: 5849486 (1998-12-01), Heller et al.
patent: 6051380 (2000-04-01), Sosnowski et al.
patent: 6093370 (2000-07-01), Yasuda et al.
patent: 6287517 (2001-09-01), Ackley et al.
patent: 6325904 (2001-12-01), Peeters
patent: 6942970 (2005-09-01), Isola et al.
patent: WO 95/12808 (1995-05-01), None
patent: WO 96/01836 (1996-01-01), None
patent: WO 98/10273 (1998-03-01), None
patent: WO 01/51665 (2001-07-01), None
patent: WO 01/73123 (2001-10-01), None
patent: WO 01/83674 (2001-11-01), None
patent: WO 02/18643 (2002-03-01), None
patent: WO 02/46472 (2002-06-01), None
Laibinis, Paul E. et al, “Orthogonal Self-Assembled Monolayers: Alkanethiols on Gold and Alkane Carboxylic Acids on Alumina”, Science, vol. 245, Aug. 25, 1989, pp. 845-847.
Sharma, Chandra P. et al,Albumin adsorption on to aluminium oxide and polyurethane surfaces, Biomaterials 1990, vol. 11, May, pp. 255-257.
Taton, T. Andrew et al, “Scanometric DNA Array Detection with Nanoparticle Probes”, Science, vol. 289, Sep. 8, 2000, pp. 1757-1760.
Michael Nehls et al., “S-300 Gel Matrix Irreversibly Binds Single-Stranded Nucleic Acids”, Trends in Genetics, 1993, p. 336-337, vol. 9(10).
Rosemary Polsky-Cynkin et al., “Use of DNA Immobilized on Plastic and Agarose Supports to Detect DNA by Sandwich Hybridization”, Clinical Chemistry, 1985, p. 1438-1443, vol. 31(9).

Affiliated with

Also associated with

Rating

Apparatus and methods for detecting DNA in biological samples does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Apparatus and methods for detecting DNA in biological samples, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus and methods for detecting DNA in biological samples will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3719886