Apparatus and methods for chemical synthesis

Details Apparatus and methods for chemical synthesis Apparatus and methods for chemical synthesis

2005-03-15

2005-03-15

Warden, Jill (Department: 1743)

Chemistry: analytical and immunological testing

Including sample preparation

Volumetric liquid transfer

C422S105000, C422S051000, C422S068100, C422S081000

Reexamination Certificate

active

06867050

ABSTRACT:
Apparatuses and methods are described for parallel oligonucleotide synthesis of hundreds of different sequences and lengths at a time. Standard phosphoramidite chemistry is employed. The syntheses take place in a reaction plate compatible with the industrial standard microplate format to allow the use of readily available automated instruments for subsequent processing. Key parameters in reducing synthesis volume in small reaction wells are discussed. This invention provides solutions to the difficulties of low volume, high number synthetic reactions.

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